Opening the Gate to Super-Resolution Leica TCS SP8 STED
Studying subcellular architecture and dynamics with conventional fluorescence microscopy can be challenging as structures smaller than 200 nm are lost. The
Leica TCS SP8 STED super-resolution microscope allows you to discover more and provides fast, intuitive, purely optical access to structural
details smaller than 50 nm. This means:
Colocalization studies and live cell imaging – at the nanoscale!
STED microscopy is a stunningly simple way to overcome the diffraction limit - where fluorescence is generated is scaled down to sub-diffraction size. Stimulated emission switches off the ability of the dye to fluoresce in the periphery of the excitation spot yielding confocal super-resolution pixel by pixel
• Resolve details smaller than 50 nm – quickly and directly
Just one click instantly switches from confocal to super-resolution, even during a live scan. Within seconds, imaging parameters can be optimized and super-resolved images obtained. STED microscopy does not rely on mathematics.
What you see is what you get! Of course, results can be improved further by deconvolution.
A Leica TCS SP8 STED is not only a super-resolution microscope but at the same time a highly versatile an upgradable confocal platform. Superior optics combined with multispectral Hybrid Detection maximizes the number of collected photons. This yields super-resolved images of optical sections, even from weakly stained samples. Allow your Leica TCS SP8 to grow – upgrade to STED! (see image 1)
• Study dynamics in living cells – at the nanoscale
STED microscopy minimizes the effect of sample drift and combines intrinsic optical sectioning capability and fast image acquisition. The applicability of fluorescent proteins further qualifies STED imaging to reveal fast dynamic processes inside living cells or even organisms, with unmatched precision.
The gated STED option – the latest milestone in confocal super-resolution – even extends the functionality of the proven STED CW substantially. Combining the superior Leica Hybrid detection and White Light Laser, gated STED improves resolution and contrast attained, while distinctly reducing laser intensity. This enhances photostability as well as the capability for live cell imaging. Acquire STED-images 12 µm inside a living drosophila larvae featuring a thick cuticula!
Discover what´s inside – with confocal super-resolution! (see image 2)
• Perform colocalization studies – with dual color STED imaging "This is the end of
colocalization analysis as we know it"
(Prof. P. McPherson, Montreal
Neurological Institute of McGill
STED gives immediate access to
dual color super- resolution images. The
STED doughnut is used for both
quasi- simultaneously acquired colors.
This empowers colocalization studies at
the nanoscale without the need to
compensate for chromatic aberrations or
Seeing how two
structures are organized in relation to
one another in sub- diffraction detail
opens the door to a completely new world
of colocalization studies.(see image 3)
• Enjoy the freedom to choose – popular fluorescent dyes and proteins
microscopy works with standard
fluorophores such as Alexa 488, Oregon
Green 488 and
FITC and does not rely on
oxygen depletion or reducing agents in
the embedding medium. Also eYFP, Citrin
and eGFP yield convincing results.
This allows you to stay as close as
possible to routine protocols saving
time and money. (see image